Microalgae have become important for biotechnological applications and so it has become important to cryopreserve the viability and functionality of microalgae strains. Studies have taken premeditated approaches to increase cryopreservation success, one such approach is to conduct CPA tolerance/toxicity tests on microalgae strains. This study tested the CPA tolerance of two Prymnesium parvum strains by exposing them to different CPA concentrations and different equilibration periods. The strains are cultured until the first day of stationary phase, and harvested for CPA exposure tests. Results proved that there are intraspecific differences in cryopreservation success, and this is due to a myriad of factors influencing cryopreservation success.
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